rabbit anti-pitx3 (Thermo Fisher)
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 
90
Structured Review
Thermo Fisher
rabbit anti-pitx3
Rabbit Anti Pitx3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-pitx3/pmc11144150-57-58-61
Average 90 stars, based on 1 article reviews
Rabbit Anti Pitx3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-pitx3/pmc11144150-57-58-61
Average 90 stars, based on 1 article reviews
rabbit anti-pitx3 - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease"
Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease
Journal: Neurochemical Research
doi: 10.1007/s11064-024-04130-y
Figure Legend Snippet: Effects of miR-124-3p in the expression of dopaminergic neuronal markers in the SN and striatum of a rat PQ model in vivo. (a) Design and timeline of the experimental animal procedure. Male Wistar rats were subjected to intranigral injection with 250 nM miR-124-3p, or saline, followed by four i.p injections of saline or PQ (10 mg/kg), separated by one day. Five days after the last PQ injection, the brains were collected for western blot analysis. Expression levels of TH in the (b) SN and (c) striatum, and (d) PITX3 in the SN of adult Wistar rats treated with saline, PQ or 250 nM miR-124-3p + PQ. (e) Representative western blot protein bands of PITX3 (32 kDa) and actin (42 kDa). TH and PITX3 protein levels were normalized against actin. Data are expressed as a percentage of the controls (saline) ± SEM. Protein expression in the control condition (saline) was set to 100%. N = 3 or 4 rats. * p < 0.05 compared to the saline group and ## p < 0.01 compared to the PQ-treated group using the one-way ANOVA followed by Sidak’s multiple comparison test. Abbreviations: i.p, intraperitoneal; TH, tyrosine hydroxylase; PITX3, pituitary homeobox 3; PQ, paraquat; SN, substantia nigra
Techniques Used: Expressing, In Vivo, Injection, Saline, Western Blot, Comparison
Related Articles
Electrophoresis:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Membrane:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Incubation:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Expressing:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and In Vivo:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Injection:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Saline:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Western Blot:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Comparison:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Fluorescence:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Derivative Assay:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Staining:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Immunofluorescence:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Marker:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Quantitative RT-PCR:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Isolation:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Transplantation Assay:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Laser Capture Microdissection:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Microarray:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Modification:Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson's Disease. Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling Article Snippet: Mounted sections were post-fixed in acetone at −20°C, dried at 40°C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and Article Title: Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons Article Snippet: The following primary antibodies were used: sheep/rabbit anti-TH (1∶1,000; Pel-Freez Biologicals), Article Title: MicroRNA-124-3p Modulates Alpha-Synuclein Expression Levels in a Paraquat-Induced in vivo Model for Parkinson’s Disease Article Snippet: Then, 30 μg of total protein was loaded in a 12.5% or 15% polyacrylamide gel, followed by electrophoresis and transfer onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK) for 90 min. Membranes were blocked using 5% milk or 5% bovine serum albumin and then incubated with the following primary antibodies: mouse anti-TH (1:5000; BD Biosciences), Article Title: TGFβ3, dibutyryl cAMP and a notch inhibitor modulate phenotype late in stem cell-derived dopaminergic neuron maturation Article Snippet: Cells were blocked with 3% (v/v) donkey serum in permeabilization solution 2 following incubation with primary Article Title: Generation of neural stem cells from human trophoblast stem cells Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin28KD (1:1000: Sigma), rabbit anti-c-Fos (1:5000, Calbiochem), chicken anti-GFP (1:1000; Abcam), rabbit anti-GFP (1:20 000; Abcam), rabbit anti-GIRK2 (1:100; Alomone Labs, Jerusalem, Israel) Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells Article Snippet: Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R&D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R&D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), Article Title: Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling. Article Snippet: Mounted sections were post-fixed in acetone at −20◦C, dried at 40◦C for 1 min, washed twice with cold 2 M NaCl PBS, permeabilized with 0.25% Triton X-100 in 2 M NaCl PBS for 2 h, incubated with primary antibodies [rabbit anti-PITX3 1:100; Thermo Fisher Scientific 38-2850; chicken anti-b-Galactosidase (b-GAL) 1:1000; abcam ab9361] overnight and |
